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Auteur (up) Trochet, D.; Mergui, X.; Ivkovic, I.; Porreca, R.M.; Gerbault-Seureau, M.; Sidibe, A.; Richard, F.; Londono-Vallejo, A.; Perret, M.; Aujard, F.; Riou, J.-F. url  doi
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  Titre Telomere regulation during ageing and tumorigenesis of the grey mouse lemur Type Article scientifique
  Année 2015 Publication Biochimie Revue Abrégée Biochimie  
  Volume 113 Numéro Pages 100-110  
  Mots-Clés Ageing; Grey mouse lemur; Primates; Senescence; Single telomere length amplification; Telomere  
  Résumé Telomere erosion leading to replicative senescence has been well documented in human and anthropoid primates, and provides a clue against tumorigenesis. In contrast, other mammals, such as laboratory mice, with short lifespan and low body weight mass have different telomere biology without replicative senescence. We analyzed telomere biology in the grey mouse lemur, a small prosimian model with a relative long lifespan currently used in ageing research. We report an average telomere length by telomere restriction fragment (TRF) among the longest reported so far for a primate species (25-30 kb), but without detectable overall telomere shortening with ageing on blood samples. However, we demonstrate using universal STELA (Single Telomere Length Amplification) the existence of short telomeres, the increase of which, while correlating with ageing might be related to another mechanism than replicative senescence. We also found a low stringency of telomerase restriction in tissues and an ease to immortalize fibroblasts in vitro upon spontaneous telomerase activation. Finally, we describe the first grey mouse lemur cancer cell line showing a dramatic telomere shortening and high telomerase activity associated with polyploidy. Our overall results suggest that telomere biology in grey mouse lemur is an exception among primates, with at best a physiologically limited replicative telomere ageing and closest to that observed in small rodents.  
  Adresse Structure et Instabilite des Genomes, Sorbonne Universites, Museum National d'Histoire Naturelle, Inserm U 1154, CNRS UMR 7196, CP26, 57 rue Cuvier, 75005 Paris, France. Electronic address: riou@mnhn.fr  
  Auteur institutionnel Thèse  
  Editeur Lieu de Publication Éditeur  
  Langue English Langue du Résumé Titre Original  
  Éditeur de collection Titre de collection Titre de collection Abrégé  
  Volume de collection Numéro de collection Edition  
  ISSN 0300-9084 ISBN Médium  
  Région Expédition Conférence  
  Notes PMID:25882681 Approuvé pas de  
  Numéro d'Appel CNRS @ aujard @ collection 1146  
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